Preparation and separation of d(pT)-10-n oligonucleotides.
نویسندگان
چکیده
A series of oligomers having the general formula d(pT)-10-n, n varying from 2 to 20, has been prepared by enzymatic joining of d(pT)-10, annealed on poly dA, employing T-4 polynucleotide ligase. The oligomers could be separated on 8 or 12% polyacrylamide gels. Such oligomers may prove useful as molecular weight markers and initiators for various polymerases.
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ورودعنوان ژورنال:
- Nucleic acids research
دوره 2 3 شماره
صفحات -
تاریخ انتشار 1975